For spontaneously or targeted mutant mice, this notation makes perfect sense: “+” indicates the wildtype allele, and “-” the mutant allele. Thus, we designate our mouse genotypes “-/-” for homozygous mutants, “+/-” for heterozygotes, and “+/+” for wildtypes.
What is the genotype of a mouse?
This genotype is called a “homozygous knockout,” or more commonly just “knockout” for short. Conversely, a normal mouse with two functional copies is called a “homozygous wildtype,” or just “wildtype.” Finally, mice with just one functional copy are referred to as “heterozygous,” or “hets.”
How do you write genotypes?
The genotype is often written as YY or yy, for which each letter represents one of the two alleles in the genotype. The dominant allele is capitalized and the recessive allele is lower case.
How do you genotype a rat?
Genotype can be determined by analysis of DNA extracted from tissues of young rodents. Analysis by the Polymerase Chain Reaction (PCR) requires the least amount of DNA which is easily obtained from ear punches or tail biopsy (1-7) although alternative methods are available (4,6,7).
Why do we genotype mice?
An essential step in mouse breeding is genotyping your mice to ensure that they have the expected genotype. The genotype for a specified gene in a mouse can be homozygous, heterozygous, wild type, and sometimes hemizygous (such as X-linked genes or transgenes).
What does Flox mean in mice?
HTT flox/flox (or HTT Fl/Fl) refers to the idea that the mice mentioned above have a knockin of the HTT gene in which portions of the gene are flanked by two Cre recombinase recognition sites.
What is genotype and example?
The term just means “the genes a particular organism has.” Any example of a genotype would just be a chart of a particular living thing’s chromosomes, or DNA molecules responsible for various genetic traits.
What is a genotype simple definition?
Genotype: The genetic constitution (genome) of a cell, an individual, or an organism. The genotype is distinct from the expressed features, or phenotype, of the cell, individual, or organism. The genotype of a person is that person’s genetic makeup. It can pertain to all genes or to a specific gene.
What are the 3 types of genotypes?
There are three types of genotypes: homozygous dominant, homozygous recessive, and hetrozygous.