ATP-dependent chromatin remodeling is performed by enzymes—chromatin remodeling complexes. The united activity of these enzymes forms the dynamic properties of chromatin during different nuclear processes such as transcription, replication, DNA repair, homological recombination, and chromatin assembly.
What is the function of ATP dependent chromatin remodeling complexes quizlet?
Briefly describe 3 ways that ATP-dependent chromatin remodeling complexes may change chromatin structure. ATP-dependent chromatin remodeling may effect position of nucleosomes, evict histones, and may replace histones with histone variants. The addition of methyl groups to bases of DNA after DNA synthesis.
Do chromatin remodeling complexes use ATP?
The major activities that are involved in nucleosome structure alterations use the energy supplied by ATP hydrolysis to affect nucleosomes. These enzymes are called ATP-dependent chromatin (or nucleosome) remodeling factors.
What is the function of chromatin remodeling complexes?
Chromatin remodeling complexes are multi-protein assemblies containing an ATPase subunit of the Snf2 subfamily that is capable to mobilize the nucleosomes using the energy of ATP hydrolysis and thereby alter the chromatin structure .
Which chromatin remodeling complexes increase transcription and include an ATPase subunit?
Currently, there are four chromatin remodeling complex subfamilies based on their associated ATPase: (Brahma) SWI2/SNF2, imitation switch (ISWI), Mi-2, and IN080. These allow chromatin remodeling and enable the constant switching between euchromatin and heterochromatin.
How do chromatin remodeling complexes work quizlet?
How do chromatin-remodeling complexes work? A. They use the energy from ATP hydrolysis to alter nucleosomes and make certain regions of the DNA more accessible to other proteins. … They use the energy from ATP hydrolysis to alter nucleosomes and make certain regions of the DNA more accessible to other proteins.
Which of the following are key differences between eukaryotic and prokaryotic transcriptional regulation quizlet?
Which of the following are key differences between eukaryotic and prokaryotic transcriptional regulation? Combinatorial control of gene expression is more common in eukaryotes. The complexity of eukaryotic development requires that transcription be more coordinated and integrated than in prokaryotes.
Does chromatin have RNA?
Whether RNA itself plays a direct structural role in chromatin is, however, not known. Here, we report results indicating that RNA plays a general structural role in eukaryotic chromatin. Our results indicate that purified chromatin contains significant amounts of RNA (2%–5% of total nucleic acids).
What is the difference between chromatin and chromatid?
As mentioned above, chromatin is composed of DNA and histones that are packaged into thin, stringy fibers. The chromatin undergoes further condensation to form the chromosome. … A chromatid is either of the two strands of a replicated chromosome. Chromatids connected by a centromere are called sister chromatids.
How is chromatin remodeling determined?
Nucleosome mobilization by chromatin remodeling factors is easily detected by observing band disappearance in gel, which in turn provides evidence for histone octamer displacement.
Which of the following is the chromatin remodeling complex?
Such remodeling is principally carried out by 1) covalent histone modifications by specific enzymes, e.g., histone acetyltransferases (HATs), deacetylases, methyltransferases, and kinases, and 2) ATP-dependent chromatin remodeling complexes which either move, eject or restructure nucleosomes.
What is the difference between methylation and acetylation?
Histone acetylation occurs at lysine residues and it increases gene expression in general. (B) Histone methylation: Methylation is catalyzed by histone methyltransferase. Histone demethylase reverses methylation. Methylation activates or represses gene expression depending on which residue is methylated.