You asked: Can Cre be heterozygous?

Approximately 25% of the progeny from this mating will be homozygous for the loxP-flanked allele and hemizygous/heterozygous for the cre transgene. These will be your experimental mice.

Can Cre be homozygous?

Cre alone can produce a phenotype

Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygous Cre genotypes might minimize unintended consequences of random transgene insertion. … Cre can damage DNA in fibroblasts, gastric cells, and sperm in the absence of any floxed sequence.

How do you identify Cre expressions?

There are several ways to do this:

  1. Check for cre mRNA transcripts using quantitative RT-PCR or Northern analysis of mRNA harvested from the target tissue. …
  2. Evaluate Cre protein via immunohistochemistry (IHC) or by immunoblot.

Is Cre endogenous?

The Cre enzyme can be expressed in many different organisms such as plants, bacteria, mammals, yeast. … As mammalian genomes are on average in the region of 3×109 bp there is a very low chance of finding an endogenous loxP site. For Cre to be functional in a foreign host, exogenous loxP sites must be engineered.

Can you cross two Cre lines?

Given that Vav1-Cre is expressed in the ovaries and testes, there is no way to generate both wild-type (floxed) and mutant (cre-recombined) offspring with control littermates.

How was Cre lox discovered?

Sternberg discovered the recombinase, Cre, and its specific locus of crossover, lox, while studying the maintenance of bacteriophage P1 as a stable plasmid. Recombinations mediated by Cre assist in cyclization of the DNA of infecting phage and in resolution of prophage multimers created by generalized recombination.

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Why is Cre recombinase so useful?

The Cre/loxP site-specific recombination system has emerged as an important tool for the generation of conditional somatic mouse mutants. … The spatial regulation of recombination can be achieved by using cell type-specific promoters that drive expression of Cre in the cell population or tissue of interest.

What is the meaning of Cre?

Carbapenem-resistant Enterobacterales (CRE) are multidrug-resistant organisms that that can cause serious infections and require interventions in healthcare settings to prevent spread.

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