Which stain is used to stain the chromosomes during cytological preparation?

Acetocarmine is a DNA specific stain that stains freshly prepared unfixed super coiled chromosomes during different mitotic stages; option B is correct.

Which dye is best suited for staining chromosomes?

Caramine is best suited for staining chromosomes.

Carmine is the bright red dye that is mainly used for color food, textiles and cosmetics.

What are the stains used to stain chromosomes and chromatin?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

Which stain is not used for chromosome staining?

(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.

Why is Aceto Orcein stain used?

Treatment with acid and heat is used to break up the cellulose cell wall allowing stain to permeate the tissue and makes it easier to squash the tissue on a microscope slide. Aceto-orcein stain turns chromosomes a purple-red colour.

What are the 5 steps to making a chromosome spread?

Terms in this set (6)

  1. Add cell sample to the culture media, a sterile solution that helps the cells grow.
  2. Culture, or grow the cells in a lab, for up to two weeks.
  3. Arrest, or halt, cells in metaphase. …
  4. Swell and drop cells onto microscope slides. …
  5. Stain with Giemsa dye and observe the chromosomes under a microscope.
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Which stain is usually used to Colour chromosomes Class 11?

Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.

What is the purpose of chromosome staining?

After cell culture and spreading of the metaphases onto slides, the chromosomes are stained to produce unique banding patterns or to reveal specialized structures. Molecular techniques have been developed to identify submicroscopic rearrangements and to compare karyotypes of different species.

Which stain is used for plant cell?

Complete answer: Methylene blue: Methylene blue is a common laboratory stain and is used for many different types of cells.

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