What is a chromosomal stain?

In chromosome staining, fluorochromes are used when the chromosomes are to be studied with a fluorescent microscope. They are also capable of producing bands in the chromosomes. Although fluorochromes are less stable than visible light dyes such as giemsa, as discussed earlier, they offer several advantages in banding.

What causes dark bands on chromosomes?

The dye stains regions of chromosomes that are rich in the base pairs Adenine (A) and Thymine (T) producing a dark band.

What causes chromosome banding?

Chromosome banding refers to alternating light and dark regions along the length of a chromosome, produced after staining with a dye.

What is a chromosomal material?

In the nucleus of each cell, the DNA molecule is packaged into thread-like structures called chromosomes. Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure. … DNA and histone proteins are packaged into structures called chromosomes.

What happens if a karyotype test is abnormal?

Abnormal karyotype test results could mean that you or your baby have unusual chromosomes. This may indicate genetic diseases and disorders such as: Down syndrome (also known as trisomy 21), which causes developmental delays and intellectual disabilities.

Why is Aceto Orcein stain used?

Treatment with acid and heat is used to break up the cellulose cell wall allowing stain to permeate the tissue and makes it easier to squash the tissue on a microscope slide. Aceto-orcein stain turns chromosomes a purple-red colour.

THIS IS INTERESTING:  What is phenotypic screening in drug discovery?

What does colcemid do to chromosomes?

Colcemid binds tubulin rapidly in comparison to colchicine which binds tubulin relatively slowly (2). Colcemid is used in chromosome analysis during lymphocyte karyotyping and in amniotic fluid cell chromosome analysis by preventing spindle formation during mitosis, causing metaphase arrest.

What causes a dark band on the chromosome and what is this called?

One of the basic chromosomal banding patterns is that produced by Giemsa reagent, a DNA stain applied after mild proteolytic digestion of the chromosomes. This reagent produces patterns of light-staining (G-light) regions and dark-staining (G-dark) regions.

What is a chromosome banding pattern?

Chromosome banding refers to alternating light and dark regions along the length of a chromosome, produced after staining with a dye. A band is defined as the part of a chromosome that is clearly distinguishable from its adjacent segments by appearing darker or lighter with the use of one or more banding techniques.

All about hereditary diseases